西安电子科技大学物理与光电工程学院，陕西 西安 710071
利用差动激光多普勒探测法，研究了能够探测尾流中气泡和湍流分布的光学诊断方法。实验研究了模拟尾流场中运动气泡及湍流的后向散射光的多普勒频移谱特性。采用差动相干探测法获得了模拟尾流场的流速，测量值与实际流速误差小于1.5％。实验结果表明：相干光束夹角对散射光频移量具有明显影响，散射光多普勒频移量随两相干光夹角的增大而增大；气泡运动引起的频移主要集中在低频端，为一宽带谱；并且频移谱的宽度随气泡数密度的增大而增大。频谱的这一特征可作为舰船尾流识别的判据之一。
尾流检测 激光多普勒技术 后向散射 detection of simulation wake laser Doppler technique backscattering 红外与激光工程
2015, 44(12): 3525
Author Affiliations
Abstract
1 Department of Physics, Fudan University, Shanghai 200433
2 State Key Lab for Advanced Photonic Materials and Devices, Fudan University, Shanghai 200433
3 Department of Macromolecular Science and Key Laboratory of Molecular Engineering of Polymers, Fudan University, Shanghai 200433
Water-soluble CdTe quantum dots (QD600) were synthesized by hydrothermal route. The uptake of QD600 in living cells of euglena gracilis was studied. The luminescence spectra show that the QD600 were bound in cells after incubation. The fluorescence images, measured by confocal laser scanning microscope, demonstrate that the QD600 can penetrate into the cells. The amount of cell-bound QD600 increased with incubation time of QD600 at 25 Celsius deg., but no detectable QD600 were found in the cells when the incubation temperature was 4 Celsius deg., it was suggested that the QD600 were actively taken into the cells by endocytotic pathway. These results indicate that the water-soluble CdTe quantum dots have the potential in the application of bio-labeling.
040.5570 Quantum detectors 260.3800 Luminescence 000.1430 Biology and medicine Chinese Optics Letters
2005, 3(0s): 216
Author Affiliations
Abstract
1 Department of Physics, Fudan University, Shanghai 200433
2 State Key Lab for Advanced Photonic Materials and Devices, Fudan University, Shanghai 200433
3 Department of Macromolecular Science and Key Laboratory of Molecular Engineering of Polymers, Fudan University, Shanghai 200433
Hydrophilic photo luminescent semiconductor quantum dots (QDs) are novel nanometer-size probes, which may have potential using in bio-imaging for biological objects. In this work, the photo-stability of these QDs in two kinds of living cells was studied, compared with conventional biological probes such as fluorescein isothiocyanate (FITC) and green fluorescence protein (GFP). It was found that the concentration of QDs in living cells is the dominant factor for its photo-stability in biological environment. When the concentration of the intracellular QDs was high, the QDs show good photo-stability that is much better than the organic fluorescent probes. However when its concentration was low, the QDs also can be photo-bleached quickly. Thus the reaching of the certain concentration level is the critical condition for QDs in the application of bio-imaging.
110.0110 Imaging systems 170.0170 Medical optics and biotechnology 180.0180 Microscopy Chinese Optics Letters
2005, 3(0s): 208
用激光诱发荧光的方法研究了单支线TEA CO2激光解离CCl3F所产生的CClF和C2自由基的电子激发态辐射寿命及自由基浓度的时间特性。
用TEA CO2激光对CCl3F进行红外多光子解离.用染料激光诱发荧光和最终产物的红外光谱分析方法探测了解离中间产物Cl和CClF.解离的最终产物为CCl4、CCl2F2及C2Cl2F2的三个同分异构体.对解离过程和形成最终产物的可能反应通道进行了分析.
